New Invention: Production of lutein standard from pumpkin callus.

One inventor from Malaysia have presented at the IENA Invention Fair 2013 his invention:


Production of lutein standard from pumpkin callus.”




Characterization and quantification of carotenoid compound is complicated costly and time consuming. The accuracy and reliability of the data depend solely on the standard and to High Performance Liquid Chromatography (HPLC) analysis. However , among the major constraints are to acquire and to maintain the pure standards. Carotenoid standards are commercially available but they are expensive and are prone to isomerization and oxidation. Thus, the purpose of this study is to establish a method to produce lutein by using pumpkin. (Cucurbita moshata) callus to be used as one of the carotenoid standards for determination of individual carotenoid. Pumpkin callus were treated with two types of hormones – Biphenyl-Amino Pureen (BAP) and Naphtelenic Acetic acid (NAA). The bioactive compounds detected in pumpkin callus were lutein, chlorophyl and beta carotene. This study was ranged from 0,42 mg/g to 2.14 mg/g with the purity ranging from 93% to 98%. The standard curves with five different concentrations of lutein from pumpkin callus in triplicate were constructed by plotting the peak area against the concentration. The coefficient of correlation was 0.9794. Therefore , this study established that pumpkin callus can be an alternative research tools to produce lutein compound.


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